The diagnosis of male fertility is based on microscopic assessment and analysis of sperm concentration, motility and morphology as routine indicators of human semen quality. Successful pregnancy requires a sperm with genetic integrity. Several studies have shown that male fertility is affected by sperm DNA damage. Subsequently, the assessment of DNA integrity in sperm and the study of its consequences have received considerable attention. Several clinical studies have examined the relationships between sperm DNA damage and reproductive outcomes in the context of natural and ART pregnancies. For IVF and ICSI, the true utility of sperm DNA damage assays remains to be firmly established, since the available studies are generally small, heterogeneous, with conflicting results. Current data suggest that impaired sperm DNA integrity may have the greatest effect on IUI pregnancy rates, further prospective studies are needed before testing becomes a routine part of patient management.

Background: Infertility is estimated to affect up to 15% of couples of reproductive age, and male factors contribute to 50% of these cases. Globozoospermia syndrome is a severe form of teratozoospermia, with an incidence of less than 0.1%. It is characterized by round sperm head.The most prominent feature of globozoospermia is the malformation of the acrosome, which is totally absent in the total globozoospermia. The aim of this study was to evaluate the levels of DNA FRAGMENTATION in ejaculated sperm of men with total globozoospermia.Materials and Methods: Sperm samples were obtained from 15 patients with total globozoospermia and 30 men with normozoospermia (normal morphology>4% and motility> 40%) referred to Royan institute, Tehran. Semen analysis was carried out to WHO criteria. DNA FRAGMENTATION was assessed using sperm chromatin structure assay (SCSA).Results: The patients with total globozoospermia had a significantly greater percentage of sperm with DNA FRAGMENTATION compared with that found in normozoospermic control (p<0.001).Conclusion: Several studies have attempted to correlate DNA FRAGMENTATION rate with sperm characteristics.However, there is just some case reports about DNA FRAGMENTATION rate in globozoosprmic men. We have found an association between globozoospermia and DNA FRAGMENTATION that because of remarkable patient’s number can be reliable.